Table 1.
Optimization of chitosan microspheres with application of formulation variables.
| Formulation code | Polymer (mg) | Drug (mg) | Drug: Polymer ratio | HA (mg) | Stirring rate | Cross linker Concentration (mg) | Time of cross linking (h) | Encapsulation efficiency (%) | Drug loading (%) (w/w) |
|---|---|---|---|---|---|---|---|---|---|
| F01 | 50 | 100 | 2:1 | 0 | 350 ± 25 | 100 | 12 | 41.3 | 16.61 |
| F02 | 50 | 50 | 1:1 | 0 | 350 ± 25 | 100 | 12 | 58.1 | 14.53 |
| F03 | 50 | 25 | 1:2 | 0 | 350 ± 25 | 100 | 12 | 65.8 | 9.4 |
| F04 | 50 | 25 | 1:2 | 100 | 350 ± 25 | 100 | 6 | 41.48 | 3.8 |
| F05 | 50 | 25 | 1:2 | 100 | 350 ± 25 | 100 | 12 | 77.11 | 7.01 |
| F06 | 50 | 25 | 1:2 | 100 | 350 ± 25 | 100 | 24 | 76.67 | 6.97 |
| F07 | 50 | 25 | 1:2 | 100 | 350 ± 25 | 50 | 12 | 21.35 | 2.37 |
| F08 | 50 | 25 | 1:2 | 100 | 350 ± 25 | 100 | 12 | 77.11 | 7.01 |
| F09 | 50 | 25 | 1:2 | 100 | 350 ± 25 | 150 | 12 | 80.68 | 6.21 |
| F10 | 50 | 25 | 1:2 | 100 | 250 ± 25 | 100 | 12 | 72.31 | 5.23 |
| F11* | 50 | 25 | 1:2 | 100 | 350 ± 25 | 100 | 12 | 77.11 | 7.01 |
| F12 | 50 | 25 | 1:2 | 100 | 450 ± 25 | 100 | 12 | 27.1 | 2.46 |
*F11 is the optimized formulation.