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. 2021 May 13;22:346. doi: 10.1186/s12864-021-07656-5

Table 1.

Quality assessment metrics of unfiltered and filtered transcriptome assemblies for multiple tissue types collected from four marine turtle species

Loggerhead - blood Hawksbill - blood Green turtle - blood Leatherback - blood Leatherback - brain Leatherback - lung Leatherback - ovary
raw filtered raw filtered raw filtered raw filtered raw filtered raw filtered raw filtered
Total trinity transcripts 132,146 77,392 280,711 220,458 489,355 376,736 347,717 276,709 216,942 140,332 243,118 165,611 163,840 119,574
Contig N50 3032 2552 3143 2276 3221 2303 2867 2187 3618 2788 3288 2526 3050 2373
Median contig length 675 707 574 529 606 575 597 553 666 629 632 601 673 593
Mean mapping rates
Conspecific samples 91.50% 75.36% 95.53% 93.58% 94.88% 93.94% 95.49% 94.95% 92.98% 83.22% 92.52% 82.02% 94.96% 93.89%
Heterospecific samples 82.65% 69.54% 88.56% 85.44% 86.24% 85.99% 83.58% 83.14% N/A N/A N/A N/A N/A N/A
Transrate scores
Assembly score 0.23 0.35 0.25 0.36 0.29 0.42 0.26 0.37 0.21 0.31 0.21 0.29 0.20 0.29
Optimal assembly score 0.35 0.36 0.36 0.37 0.42 0.43 0.36 0.38 0.33 0.32 0.30 0.30 0.30 0.30