. 2021 Apr 29;12:678054. doi: 10.3389/fphys.2021.678054

TABLE 1.

Least square means (LSM), their standard errors (SE) and significant differences between the effect levels for blood concentrations of total and ionized calcium, inorganic phosphate (PO4), the carboxyterminal crosslinked telopeptide of type I collagen (CTX-I), osteocalcin, 25-hydroxycholecalciferol (25(OH)D₃) and estradiol-17β of the blood of brown-(BLA) and white-egg (WLA) laying hens examined in the 17th week of age.

Effect	Total calcium (mmol/l)	Ionized calcium (mmol/l)	PO4 (mmol/l)	CTX-I (ng/ml)	Osteocalcin (ng/ml)	25(OH)D₃ (ng/ml)	Estradiol-17β (pg/ml)

	LSM ± SE	LSM ± SE	LSM ± SE	LSM ± SE	LSM ± SE	LSM ± SE	LSM ± SE
Layer line
BLA	2.64^B±0.06	1.37^A±0.02	1.69^A±0.04	1.00^A±0.03	46.63^A±4.33	47.10^B±1.88	113.06^B±18.93
WLA	2.83^A±0.06	1.42^A±0.02	1.36^B±0.04	1.03^A±0.03	35.48^B±3.16	54.85^A±1.88	237.60^A±18.93
Time of the day
6 a.m.	2.65^A±0.07	1.39^A±0.02	1.63^A±0.04	1.15^A±0.04	37.14^A±4.27	51.03^A±2.30	183.80^A±23.18
10 a.m.	2.81^A±0.07	1.37^A±0.02	1.50^AB±0.04	0.95^B±0.04	39.62^A±4.79	52.10^A±2.30	177.37^A±23.18
2 p.m.	2.74^A±0.07	1.43^A±0.02	1.45^B±0.04	0.95^B±0.04	46.42^A±4.84	49.79^A±2.30	164.82^A±23.18

^A,BMeans within a column and effect with no common superscript differ significantly at P < 0.05.