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. 2021 Apr 29;9:645386. doi: 10.3389/fcell.2021.645386

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Copyright © 2021 Roth, Baddam, Lin, Vidal-García, Aponte, De Souza, Godziuk, Watson, Footz, Schachter, Egan, Hallgrímsson, Graf and Voronova.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Ankrd11 is upregulated with bone induction. (A) RT-qPCR analysis of Ankrd11, Ocn, Ibsp, Opn, and Alp1 mRNA expression during in vitro osteogenic differentiation of P6 calvarial osteoblast precursor cells from wild-type mice. Data was normalized to 36B4 housekeeping gene. Results are relative to d15 expression from four independent biological replicates with d15 values equated to 100%. (B) Low magnification of approximate location (blue box, H&E stain) of panels showing immunostaining for Osterix (Sp7, red, top row) and Ankrd11 (red, bottom row) in E14.5 and P0 Ankrd11^ctrl mice. Images indicate similar expression domains of Sp7 and Ankrd11 at embryonic stages with expression pattern varying at birth. DAPI (blue) was used to counterstain nuclei. n = 3. D, days; E, embryonic; P, postnatal.