Figure 3.

Ligand stimulation of multiple cell-surface receptors have minimal cell viability effect in DIPG cell lines. (A–H) Selected DIPG cell lines were treated with recombinant ligands at increasing concentrations to activate their respective receptors. The ligands used are SEMA4C, SEMA4D, PDGF-AA, PDGF-BB, PDGF-CC, ACTIVIN A, EGF, PTN, CXCL12, IL-13, and DLL4. Note: DIPG cell lines used are SF-8628, DIPG 4, DIPG 13, DIPG 17, and DIPG 24 but not all cell lines are used for all ligand treatments. Cell viability values were normalized to untreated controls.