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. 2021 Feb 28;3(1):vdab039. doi: 10.1093/noajnl/vdab039

Figure 5.

Figure 5.

Invasion activity shown by multiple recombinant ligands in different DIPG cell lines. (A) Invasion in SF-8628 (100 ng/mL) and DIPG 4 (200 ng/mL) cell lines was unchanged or decreased following EGF stimulation. (B) ACVR stimulation by ACVA ligand (100 ng/mL) showed increased effects in SF-8628, DIPG 4, and DIPG 24 cell lines. (C) CXCR4 receptor stimulation by CXCL12 ligand (100 ng/mL) decreased invasiveness in SF-8628 but increased cell invasion in DIPG 24 cell line. (D) NOTCH1 receptor ligand, DLL4 showed increased invasion in all tested DIPG cell lines (SF-8628, DIPG 4, and DIPG 24) at 10 ng/mL concentration versus controls. All experiments were performed in triplicates. The (+) serum and (+) growth factors (GF) data served as positive controls and (-) serum and (-) GF were used as negative controls. (* denotes P < .05, ** denotes P < .01, and *** denotes P < .001).