TABLE 3.
List of primers used in this study
| Category and no. | Primer name | Primer sequence 5′→ 3′a |
|---|---|---|
| Real-time PCR | ||
| P1 | BAS 3757_ftsZ RT FP | TGCCTCTAACATTGGCGTGT |
| P2 | BAS 3757_ftsZ RT RP | CAAGCGGCATCTGGTATTGC |
| P3 | BAS0102 _rpoB_RT_FP | AACTTGCGCACATGGTTGAC |
| P4 | BAS0102 _rpoB_RT_RP | CTGTCCACCGAACTGAGCTT |
| Protein purification | ||
| P5 | BAS0841 Fp (BamHI) | GGGGGATCCCCATGGCAAAGACTAACTC |
| P6 | BAS0841 Rp (XhoI) | GGGCTCGAGATTATTTTGTTGCAGGTTTTGC |
| P7 | BAS0842 Fp (BamHI) | CCCGGATCCCCATGGCAAAGACTAACTCTTAC |
| P8 | BAS0842 Rp (XhoI) | GGGCTCGAGTTATAGATTTGGGTTATTAAG |
| P9 | BAS0253_groEL_BamHI_FP | AATCCAAGGGGGTGGATCCTTATGGCAAAAG |
| P10 | BAS0253_groEL_XhoI_RP | TTAGGGCAAACTCGAGTTACATCATTCCGCCC |
| P11 | BAS1683 FP (EcoRI) | CCCGAATTCATGAAAAAAGTTATTTCTAATGTG |
| P12 | BAS1683 RP (XhoI) | CCCCTCGAGTTGTATTTTTAAGTTCTTCTTCAATGTCC |
| Complement strain generation | ||
| P13 | prkC SpeI Fp | CCACTAGTCGTGCTGATTGGAAAACGCTTAAATG |
| P14 | prkC BamHI Rp | CCGGATCCTTATTGTGTTGGATATGGTACTTCTTTG |
| P15 | prkC Promoter KpnI Fp | CCGGTACCATTGTCGGTCGTGGTACAGAAACTG |
| P16 | prkC promoter SpeI Rp | CCACTAGTATGGCTCGTCCTCTTTCTTTTTC |
| bslO overexpression strain (BAS-WT::bslO) generation | ||
| P17 | bslO O/E SpeI Fp | GCGGACTAGTCATGAAAAAAGTTATTTCTAATGTG |
| P18 | bslO O/E BamHI Rp | CCCGGATCCTTATATTTTTAAGTTCTTCTTCAATGTCC |
a
Underlined nucleotides signify restriction sites.