FIG 2.

Overlap between predicted and validated C. pneumoniae proteins translocated into the host cell cytoplasm. (A) A total of 382 C. pneumoniae proteins (∼35% of genome) were expressed in C. trachomatis in GSK-tagged forms and tested for phosphorylation of the GSK3β tag with phospho-specific antibodies. Of the 75 phospho-GSK3β-positive clones, 58 proteins were specific to C. pneumoniae, including 49 proteins whose localization during infection had not been previously determined. (B) Bioinformatic analysis of the 382 C. pneumoniae tested proteins indicated that 45 are predicted to have a T3S signal, but only 23 of these proteins were positive for translocation.