FIG 3.

Subcellular localization of novel C. pneumoniae effector candidates. HeLa cells were transfected with plasmids expressing C-terminally HA-tagged C. pneumoniae proteins. At 48 h posttransfection, cells were fixed and immunostained with mouse anti-HA, the Golgi marker GM130 (A), or the mitochondrial marker COXIV (B). HeLa cells were cotransfected with plasmids expressing C-terminally HA-tagged C. pneumoniae genes and plasmids expressing markers for the plasma membrane (DMT1-mCherry [C]), late endosomes (GFP-Rab7 [D]), smooth ER (GFP-HO1 [E]), or autophagosomes (GFP-LC3B [F]). Images were acquired with a Zeiss LSM880 confocal microscope. Thin scale bars = 10 μm, and thick scale bars = 2.5 μm. (G) Quantitation of colocalization by Pearson’s correlation coefficient. Numbers indicate standard error of the mean from 3 independent experiments with >15 cells per condition.