FIG 6.

C. trachomatis expressing CPj0678 recruits PACSIN2. (A) HeLa cells were infected with C. trachomatis expressing CPj0678 (top) or wild type (WT; bottom). After a 24-h infection, cells were fixed and stained with mouse anti-Myc (green) and rabbit anti-PACSIN2 (red) antibodies. Nuclei were stained with DAPI (blue). A thin scale bar = 10 μm, and a thick scale bar = 5 μm. (B) HeLa cells were infected with C. trachomatis expressing GSK3β/Myc-tagged GFP or CPj0678. After 24 h of infection, cells were lysed in protein lysates and mixed with mouse anti-Myc antibodies at 4°C for 16 h, and associated proteins were isolated with magnetic protein G. Precipitated samples were analyzed by immunoblotting using anti-PACSIN2 or anti-GSK antibodies. Arrowheads indicate the precipitated GFP or CPj0678 protein.