TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Descriptiona | Source or reference |
|---|---|---|
| S. flexneri strains | ||
| 2457T | Serotype 2a | 82 |
| AWY3 | 2457T virB::Tn5 Knr | 16 |
| BS103 | 2457T cured of the virulence plasmid | 83 |
| Plasmids | ||
| pJNS04 | pBAD-virB; Ampr | This study |
| pJNS10 | pBAD-gfp-12AA linker-virB; Ampr | This study |
| pJNS12 | pBAD-gfp-virB; Ampr | This study |
| pJNS13 | pBAD-gfp-virB K152E; Ampr | This study |
| pJNS18 | pBAD-gfp-virB K152E/R167E; Ampr | This study |
| pJNS22 | pACYC177 carrying 25-bp VirB-binding site derived from pBT-PicsP (36); Cmr | This study |
| pJNS24 | pACYC177 without 25-bp VirB-binding site derived from pBT-empty (36); Cmr | This study |
| pJH66 | pBAD-linker-gfp; Ampr | 79 |
| pBAD/His A | pBR322-derived expression vector allowing regulated and dose-dependent recombinant protein expression; Ampr | Invitrogen |
| pAFW04 | pACYC184-PicsP-lacZ; Cmr | 17 |
| pHJW20 | WT PicsP fused to lacZ; Cmr | 35 |
| pMIC18 | pHJW20 with a mutated VirB-binding site; Cmr | 35 |
| pJAI28 | WT PospD1 fused to lacZ; Cmr | 18 |
| pJAI36 | pJAI28 with mutated VirB-binding sites; Cmr | 18 |
| pATM324 | pBAD18-virB; Ampr | 67 |
a
Knr, kanamycin resistance; Ampr, ampicillin resistance; Cmr, chloramphenicol resistance.