TABLE 4.

Metal content of AE104 and derivate strains^a

Metal (atoms/cell)	Metal content (atoms/cell) in:
	AE104		ΔzntR mutant		ΔcadR mutant
	No addition	1 μM Cd	No addition	1 μM Cd	No addition	1 μM Cd
106 × Mg	14 ± 1.4	13 ± 1.2	12 ± 1.3	12 ± 0.8	14 ± 1.4	13 ± 1.8
103 × Fe	910 ± 33	860 ± 62	910 ± 68	900 ± 77	910 ± 67	910 ± 110
103 × Zn	70 ± 17	61 ± 7.7	65 ± 8.8	62 ± 6.2	67 ± 8.8	63 ± 6.1
103 × Co	23 ± 6.3	8 ± 2.8	25 ± 5.4	7 ± 2.3	25 ± 5.4	4 ± 0.08
1 × Cd	91 ± 27	44,000 ± 3,100	111 ± 8.4	57,000 ± 5,300	78 ± 15	35,000 ± 2,400

^aThe cells from the precultures were diluted 100-fold into fresh TMM and incubated with shaking until the turbidity of the exponentially growing cells reached 100 Klett units. CdCl₂ (1 μM) was added or not, and incubation was continued until the turbidity reached 150 Klett units. The AE104 parent, ΔcadR (ΔRmet_2302), and ΔzntR (ΔRmet_3456) cells were harvested by centrifugation, and the cellular metal content was determined by ICP-MS. Values are means and deviations for ≥3 experiments.