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. 2021 Apr 27;87(10):e02082-20. doi: 10.1128/AEM.02082-20

TABLE 3.

Changes in abundance of bacterial ASVs in the ILF and intestinum of Hirudo verbana and Macrobdella decora leeches

Host species and bacterial class Genus or familya ASVb Base meanc Log2 fold changed Adjusted Pe Location with higher abundancef
Hirudo verbana
 Bacteroidia Bacteroides ASV34 29 −9 3.8 × 10−10 ILF
  Bacteroides ASV51 11 −8 5.0 × 10−6 ILF
  Mucinivorans ASV1 4,520 −5 2.8 × 10−44 ILF
  Mucinivorans ASV10 187 −27 1.0 × 10−62 ILF
  Mucinivorans ASV23 10 8 0.008 Intestinum
  Rikenella ASV31 15 28 3.2 × 10−8 Intestinum
  Rikenella-like ASV15 25 5 1.1 × 10−7 Intestinum
 Clostridia Proteiniclasticum-like ASV7 239 −27 5.6 × 10−77 ILF
  Proteocatella ASV22 39 −26 7.9 × 10−44 ILF
 Fusobacteriia Fusobacterium ASV8 182 −28 1.6 × 10−40 ILF
 Alphaproteobacteria Rhodospirillaceae ASV11 45 10 6.4 × 10−7 Intestinum
 Gammaproteobacteria Aeromonas ASV2 820 −2 0.03 ILF
  Morganella ASV55 17 −6 2.1 × 10−5 ILF
Proteus ASV40 13 −8 2.6 × 10−6 ILF
Macrobdella decora
 Bacteroidia Bacteroides-like ASV28 41 3 0.03 Intestinum
  Bacteroides-like ASV3 1,327 0 0.99 —
  Bacteroides-like ASV5 1,110 0 0.99 —
  Mucinivorans ASV49 28 11 2.5 × 10−9 Intestinum
 Clostridia Butyricicoccus-like ASV6 763 −1 0.12 ILF
  Proteocatella ASV4 1,124 0 0.99 —
  Sporobacter-like ASV13 96 −1 0.99 ILF
  Tyzzerella ASV20 93 −3 2.1 × 10−5 ILF
 Alphaproteobacteria Insolitispirillum ASV52 13 7 1.1 × 10−4 Intestinum
  unk_Rhodospirillaceae ASV38 20 8 9.1 × 10−5 Intestinum
 Gammaproteobacteria Aeromonas ASV2 486 0 0.99 —
a

ASVs belonging to the core (present in ≥90% of samples) are highlighted in gray. Common (present in 70 to 90% of samples) ASVs are in bold. Core and common ASVS were determined for each species of leech and sampled organ individually. The ASV labeled “unk_” has no closely related genus listed in RDP.

b

ASV ID specific to this study. See Table S4 for GenBank accession numbers.

c

Mean number of reads per sample of 4,990 total reads, calculated using both ILF and intestinum samples. Median reads per sample type are reported in Table S4. The minimum read count to be considered present in a sample was 7 (see Materials and Methods).

d

Calculated with abundance in intestinum samples as the numerator and abundance in ILF samples as the denominator.

e

P values were adjusted for multiple comparisons. Values in bold are significant (P < 0.001).

f

Gastrointestinal location with higher abundance of specified ASV. —, log2 fold change of <1 between gastrointestinal locations.