TABLE 2.
Sequences of primers and probes used in this study
| Target | Primer or probea | Sequenceb |
|---|---|---|
| RVA | Rota NVP3-F1 | ACCATCTACACATGACCCTC |
| Rota NVP3-F2 | ACCATCTTCACGTAACCCTC | |
| Rota NVP3-R | GGTCACATAACGCCCC | |
| Rota NVP3-TP | FAM-TGAGCACAATAGTTAAAAGC-MGB-NFQ | |
| Rotarix | Rotarix VP4-F | TGTGAGTAA“C”GATTCAAATAAATGGAAGTT |
| Rotarix VP4-R | TCACCATGAAATGTCCATACTCTTCCACCA | |
| Rotarix VP4-P | FAM-ATA{C}CAGA{C}TTGTAGGAATAYTTAAATA-BHQ1 | |
| RotaTeq | RotaTeq-VP6-F | GCGGCGTTATTTCCAAATGCACAG |
| RotaTeq-VP6-R | CGTCGGCAAGCACTGATTCACAAA | |
| RotaTeq-VP6-P | FAM-ATCACGCAA“C”AGTAGGACT“C”ACGCTT-BHQ1 | |
a
Forward and reverse primers are indicated by F and R at the end of the designation. Probes are indicated by P at the end of the designation.
b
{C} and “C” denote AP-dC (G-clamp) and C-5 propynyl-dC, respectively. FAM, MGB, NFQ, and BHQ in the probes stand for 5-carboxyfluorescein, minor groove binder, nonfluorescent quencher, and black hole quencher, respectively.