TABLE 2.
L. monocytogenes strains and plasmids used in this study
| Strain or plasmid | Description | Mutation | Source or reference |
|---|---|---|---|
| Strains | |||
| 15G01 | Wild-type serotype 1/2a; Ems Kans | 18 | |
| 34F11 | 15G01 with transposon inserted in the clsA gene (LMON_2515); Emr Kans | 15G01 clsA::himar1 | This study |
| 33E11 | 15G01 with transposon inserted in the uvrB gene (LMON_2501); Emr Kans | 15G01 uvrB::himar1 | This study |
| 33E11-C | 33E11 containing the pIMK-uvrB plasmid; Emr Kanr | 15G01 uvrB::himar1/pIMKuvrB | This study |
| 33E11-EV | 33E11 containing the pIMK plasmid; Emr Kanr | 15G01 uvrB::himar1/pIMK | This study |
| 39G5 | 15G01 with transposon inserted in the mltD gene (LMON_2714); Emr Kans | 15G01 mltD::himar1 | This study |
| 39G5-C | 39G5 containing the pIMK-mltD plasmid; Emr Kanr | 15G01 mltD::himar1/pIMKmltD | This study |
| 39G5-EV | 39G5 containing the pIMK plasmid; Emr Kanr | 15G01 mltD::himar1/pIMK | This study |
| 44D3 | 15G01 with transposon inserted in the mgtB gene (LMON_2712); Emr Kans | 15G01 mgtB::himar1 | This study |
| 41H7 | 15G01 with transposon inserted in the flaA gene (LMON_0695); Emr Kans | 15G01 flaA::himar1 | This study |
| Plasmids | |||
| pIMK | Site-specific listerial integrative vector, 5.1 kb; Kanr | 80 | |
| pIMK-uvrB | Site-specific plasmid carrying the LMON_2501 gene; Kanr | This study | |
| pIMK-mltD | Site-specific plasmid carrying the LMON_2714 gene; Kanr | This study |