TABLE 3.
Biomass, roughness coefficient, maximum thickness, and average thickness values for of biofilms formed by WT and mutants 39G5 (mltD mutant) and 44D3 (mgtB mutant) strainsa
| Parameter | Result (SD) |
|||||
|---|---|---|---|---|---|---|
| WT | WT-Mg2+ | 39G5 | 39G5-Mg2+ | 44D3 | 44D3-Mg2+ | |
| Biomass (μm3/μm2) | 1.145 (0.054) | 0.497 (0.409) | 1.813 (1.620) | 0.191 (0.043) | 0.841 (1.130) | 1.626 (0.950) |
| Roughness coefficient (Ra*) | 0.639 (0.074) | 1.515 (0.256) | 0.891 (0.750) | 1.787 (0.009) | 1.284 (0.874) | 0.654 (0.455) |
| Maximum thickness (μm) | 4.784 (1.452) | 4.951 (0.317) | 9.162 (3.767) | 4.280 (0.00) | 10.966 (6.764) | 5.077 (0.262) |
| Avg thickness (entire area) (μm) | 2.295 (0.776) | 0.840 (0.561) | 4.063 (2.399) | 0.320 (0.089) | 3.107 (4.807) | 2.398 (1.340) |
| Avg thickness (biomass) (μm) | 3.357 (1.377) | 3.281 (0.533) | 7.408 (2.243) | 3.026 (0.913) | 4.904 (4.283) | 3.238 (1.082) |
a
The biomass, roughness coefficient, maximum thickness, and average thickness of biofilms formed by the wild type (WT) and the mutants 39G5 (mltD mutant) and 44D3 (mgtB mutant) in MWB and MWB with a final concentration of 5 mM Mg2+ after incubation at 30°C for 7 days were calculated using COMSTAT. Standard deviations were calculated from three analyzed images taken of each sample.