TABLE 4.
Primers used in this study
| Usage and primer | Primer nucleotide sequence (5′–3′)a | Product size (bp) | Source or reference |
|---|---|---|---|
| Complementation of 33E11 | |||
| uvrB_Fwd | CAACTGCAGCCTTCAATTAAATCCACATCTGGT (PstI) | 2,528 | This study |
| uvrB_Rev | AACGGATCCTGTGCTTGCAACGTATATGCT (BamHI) | This study | |
| Complementation of 39G5 | |||
| mltD_Fwd | CAACTGCAGTTGACGTAGAAACACCTTAGCAC (PstI) | 2,683 | This study |
| mltD_ Rev | AACGGATCCAAAGGCAATTTCGGTGCGAC (BamHI) | This study | |
| Identification of L. monocytogenes | |||
| 16S_Fwd | CAGCAGCCGCGGTAATAC | 938 | 87 |
| 16S_Rev | CTCCATAAAGGTGACCCT | 87 | |
| Arbitrary PCR | |||
| Marq254 | CGTGGAATACGGGTTTGCTAAAAG | 76 | |
| Marq255 | CAGTACAATCTGCTCTGATGCCGCATAGTT | 76 | |
| Marq206 | TGTCAGACATATGGGCACACGAAAAACAAGT | 76 | |
| Marq207 | GGCCACGCGTCGACTAGTACNNNNNNNNNNGTAAT | 76 | |
| Marq208 | GGCCACGCGTCGACTAGTAC | 76 | |
| Marq257 | CTTACAGACAAGCTGTGACCGTCT | 76 | |
| Marq270 | TGTGAAATACCGCACAGATGCGAAGGGCGA | 76 | |
| Marq271 | GGGAATCATTTGAAGGTTGGTACT | 76 | |
| Presence of vector pIMK | |||
| T7promoter | TAATACGACTCACTATAGGG | Macrogen, Inc. (South Korea) |
a
Italics represent restriction enzyme sites; the restriction enzymes used are indicated in parentheses. N = A, C, G, or T.