Figure 4.

DNGR-1 dampens an activation signature induced by Flt3L (FL) in tumor-infiltrating conventional type 1 dendritic cells (cDC1s). RNA-Seq from CD103⁺ dendritic cells (DCs) purified from day 13 B16F10 tumors grown in WT and Clec9a^gfp/gfp mice that were hydrodynamically injected intravenously with FL or an empty plasmid. (A) Pairwise gene set enrichment analysis (GSEA). Only significantly enriched gene sets are colored according to their normalized enrichment score (NES). Upper panel: hallmark GSEA of intratumor CD103⁺ DCs from WT and Clec9a^gfp/gfp mice comparing FL-treated versus empty plasmid (control). Lower panel: hallmark GSEA of intratumor CD103⁺ DCs from FL-treated and empty plasmid (control) comparing WT versus Clec9a^gfp/gfp mice. Representative enrichment plots are shown in online supplemental figure S5. (B) Gene expression heatmap for metaclusters of interest after k-means clustering of differentially expressed genes in intratumor CD103⁺ DCs across all four conditions. Some genes of interest are highlighted. Three independent experiments were performed with samples from n=2–3 pooled mice per experiment. (C) Z-score for each of the genes belonging to metaclusters 1 and 2 (MC1 and MC2, respectively).