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. Author manuscript; available in PMC: 2021 May 13.
Published in final edited form as: Curr Res Biotechnol. 2019 Nov 15;1:78–86. doi: 10.1016/j.crbiot.2019.11.001

Figure 1. RADD detection of DNA damage within FFPE samples.

Figure 1.

A. RADD workflow. FFPE tissue samples are deparaffinized and rehydrated. The RADD assay is performed with a cocktail of DNA repair enzymes that detects and removes damaged DNA leaving gaps where damaged bases were encountered. Lesion filling occurs with a DNA polymerase and a tagged dUTP molecule allows for fluorescent detection of the damage site. B. RADD detection of DNA lesions within FFPE immortalized cell lines. C. Strand break markers for γH2AX and 53BP-1 confirm presence of DNA damage within the FFPE samples. Scale bars 100 μm.