Spectra of two samples of MurNAc-alk obtained using the direct coupling method (1, lower spectrum) and with the NHS method (2, upper one), both purified through column chromatography. On (2), the signal at δ=2.60 ppm originated by the methylenes of the NHS shows that further purification is needed.
Template: This template should be printed at 100% size to cut the properly-sized components to make the cytocentrifuge units in Support Protocol 4.