Fig 3.

Inhibitory activity of the crude extracts obtained from endophytic fungi associated with Halopteris scoparia against hyaluronidase (A), collagenase (B), elastase (C) and tyrosinase (D) activities: inhibition of hyaluronidase is represented by the percentage of hyaluronic acid that was not degraded in the presence of 200 μg/mL of each extract; and inhibition of collagenase, elastase, and tyrosinase are represented by the interference of 200 μg/mL of each extract on their activity. The fungi from which the extracts were obtained are abbreviated as follows: Asp.che13 or 20, isolates 13 or 20 of Aspergillus chevalieri; Pen.siz, Penicillium sizovae; Pen.exi, Penicillium sect. Exilicaulis; Diap.sp, Diaporthe sp.; Nig.ory2 or 89, isolates 2 or 89 of Nigrospora oryzae; Fus.i-e, Fusarium incarnatum-equiseti complex; Art.aru, Arthrinium arundinis; Ste.gra, Stemphylium gracilariae. The values correspond to mean ± SEM of 3 independent experiences, in duplicate. * marks significant differences (One-way ANOVA, Dunnett’s test; p< 0.05) when compared with the vehicle.