Table 1. Endophytic fungi isolated from Halopteris scoparia and molecularly identified by comparison of ITS and cal, tef1-α, tub and/or his3 sequences with those available on GenBank database.
| Collection area (date) | Isolate | Identified fungal taxa | NCBI accession n° | Discriminatory loci | Cover/ Identity (%) | BLAST best hits (NCBI accession n°) |
|---|---|---|---|---|---|---|
| Gb (Jun.18) | 13 | Aspergillus chevalieri a | MW856073 | cal | 98/ 100 | Aspergillus chevalieri (MK451332) |
| AN (Aug.18) | 20 | Aspergillus chevalieri | MW856074 | cal | 100/ 100 | Aspergillus chevalieri (MK451332) |
| Bl (Dec.18) | 93 | Penicillium sizovae a | MW856079 | tub | 99/ 100 | Penicillium sizovae (LT898305) |
| Bl (Dec.18) | 92 | Penicillium sect. Exilicaulis a | MW856078 | tub | 100/ 99 | Penicillium arabicum (LT898226) |
| Gb (Jun.18) | 1A | Diaporthe sp. b | MW822676 | ITS | 100/100 | Diaporthe leucospermi (JN712460) |
| MW856070 | tef1-α | 99/ 98.5 | Diaporthe rossmaniae (MK828064) | |||
| MW856076 | tub | 97/ 99.4 | Diaporthe rossmaniae (MK837915) | |||
| MW856072 | cal | 76/ 99.8 | Diaporthe rossmaniae (MK883823) | |||
| MW856069 | his3 | 100/ 99.8 | Diaporthe rossmaniae (MK871433) | |||
| Gb (Jun.18) | 2 | Nigrospora oryzae b | MW822677 | ITS | 100/ 99.6 | Nigrospora oryzae (MH003399) |
| Bl (Dec.18) | 89 | Nigrospora oryzae | MW822678 | ITS | 100/ 100 | Nigrospora oryzae (GU073124) |
| Bl (Dec.18) | 90 | Fusarium incarnatum-equiseti complex b | MW856071 | tef1-α | 100/ 98.3 | Fusarium equiseti (DQ854856) |
| Bl (Dec.18) | 88 | Arthrinium arundinis b | MW856077 | tub | 99/100 | Arthrinium arundinis (AB220321) |
| AN (Aug.18) | 22 | Stemphylium gracilariae c | MW856075 | cal | 99/ 100 | Stemphylium gracilariae (KU850839) |
a Eurotiomycetes,
b Sordariomycetes,
c Dothideomycetes.
* BLAST best hit for the ITS sequence of isolate 1A, considering that the results from BLAST varied slightly depending on the gene compared against the NCBI database. Collection areas are abbreviated as folllows: Gb, Gambôa; AN, Portinho da Areia Norte; Bl, Baleal.