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Glycine insertion (+2 G) at position T2128 results in a trafficking defect. (a) Peak currents elicited per cell-attached patch comparing WT hPiezo1 to the T2128 + 2 G mutant at +60 mV pipette potential (p – value determined using T-test. (b) Representative western blot showing WT, P2113 + 2 G, G2163 + 2 G and T2128 + 2 G (FG – Fully glycosylated), (n = 5)
