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. 2021 May 10;43(1):803–810. doi: 10.1080/0886022X.2021.1915801

Figure 4.

Figure 4.

The inflammation was suppressed by FG-4592 in hypoxia-induced TECs. (A) qRT-PCR analysis of cytokine (TNF-α, IL-1β and CCL-2) mRNA expression levels in TECs lysates (n = 6). (B) qRT-PCR analysis of cytokine (TNF-α, IL-1β and CCL-2) mRNA expression levels in HK-2 cells. (C) Western blotting analysis of p-p65 protein in HK-2 cells. Quantitative analysis of the Western blots of p-p65. GAPDH was used as the loading control. (D) Representative immunofluorescence staining of p-p65 (×1000). HK-2 were pretreated with or without FG-4592, then cultured with H/R. GAPDH was used as the loading control. All experiments were duplicated three times. **p < .01 vs. I/RI + NC or H/R + NC group, #p < .05 vs. I/RI + NC or N/N group, ANOVA followed by Bonferroni correction.