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. Author manuscript; available in PMC: 2021 May 13.
Published in final edited form as: J Vis Exp. 2021 Apr 12;(170):10.3791/62173. doi: 10.3791/62173

Figure 2: SUMO is enriched after recruiting SIM to telomeres with dimerizers.

Figure 2:

(A) Dimerization schematic in this experiment: SIM (or SIM mutant) is fused to mCherry and eDHFR, and TRF1 is fused to Halo and GFP. (B) A representative cell for telomere DNA FISH and SUMO1 IF after recruiting SIM. Bottom is binary layer identifying telomeres, SUMO1 and number of colocalized SUMO1 and telomere DNA foci. Scale bars, 5 μm. (C) A representative cell for telomere DNA FISH and SUMO1 IF after recruiting SIM mutant. At the bottom is the binary layer of the images used to identify the number of colocalized SUMO1 and telomere DNA foci. Scale bars, 5 μm. (D) A representative cell for telomere DNA FISH and SUMO2/3 IF after recruiting SIM. At the bottom is the binary layer identifying telomeres, SUMO2/3, and the number of colocalized SUMO2/3 and telomere DNA foci. Scale bars, 5 μm. (E) A representative cell for telomere DNA FISH and SUMO2/3 IF after recruiting SIM mutant. At the bottom is the binary layer of the images used to identify the number of colocalized SUMO2/3 and telomere DNA foci. Scale bars, 5 μm.