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. Author manuscript; available in PMC: 2021 May 13.
Published in final edited form as: J Vis Exp. 2021 Apr 12;(170):10.3791/62173. doi: 10.3791/62173

Figure 4: Reversal of condensation and telomere clustering.

Figure 4:

(A) Snapshots of TRF1-GFP and SIM-mCherry after adding 100 μM TMP (final concentration) to cells with condensates formed for 3 hours. At the bottom is the telomere binary layer identified from TRF1-GFP. Scale bars, 5 μm. (B) Average telomere intensity (summarize intensity over the volume in each telomere and then average over all telomeres in a cell) over time for cell in Figure 4A. (C) Telomere number over time for cell in Figure 4A.