FIGURE 1.

In a population of fully-complementary 17 nucleotide-long guides targeting randomized-sequence single- and double-stranded substrates of various GC contents, the first position of the guide (g1) was strongly correlated with overall guided-endonuclease activity of TtAgo. In particular, guides with g1T performed better than those with g1V. Each g1 grouping is divided to show the overall GC content of the substrate targeted, however, the significance levels shown are for each g1 grouping against the others. Boxplots have been annotated for statistical significance between relevant groups using the following labels: ns (not significant) for p > 0.05; ^∗ for p ≤ 0.05; ^∗∗∗ for p ≤ 0.001; and ^**** for p ≤ 0.0001.