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. 2021 Apr 29;8:670940. doi: 10.3389/fmolb.2021.670940

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Copyright © 2021 Hunt, Tamanaha, Bonanno, Cantor and Tanner.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Guided-endonuclease activity of TtAgo using fully-complementary guides, where g1 is complementary to t1 (g1:t1), and partially complementary guides, where g1 is not complementary to t1 (g1Xt1) acting on (A) single-stranded and (B) double-stranded DNA substrates. Significance levels shown only for g1:t1 to g1Xt1 comparisons. Boxplots have been annotated for statistical significance between relevant groups using the following labels: ns (not significant) for p > 0.05; ^∗ for p ≤ 0.05, ^∗∗ for p ≤ 0.01; ^∗∗∗ for p ≤ 0.001; and ^**** for p ≤ 0.0001.