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. 2021 Apr 29;9:661759. doi: 10.3389/fcell.2021.661759

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Copyright © 2021 Gilardi and Kalebic.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Techniques that allow acute manipulation of the ferret neocortex development in vivo. (A) Pharmacological treatment. Intraperitoneal (i.p.) injection of the anti-mitotic drug methylazoxymethanol (MAM) allows to study neurogenesis and model cortical dysplasia. (B) In utero electroporation enables manipulation of gene expression in neural progenitors and their progeny during embryonic development. (C) Postnatal electroporation during the first week of postnatal life enables studies of neuronal migration. (D) Viral injection at postnatal stages applied to study neural migration. (E) An example of the ferret P0 neocortex that was targeted by in utero electroporation at E33. Immunofluorescence for GFP (green), combined with DAPI staining (blue). Scale bar, 500 μm. This image has been modified from Kalebic et al. (2018).