Figure 3.

dmLNP-siRNAs characterization and biodistribution

(A) Molar composition of dmLNP-siRNA LNPs. (B) Nanoparticle size distribution of dmLNP-siRNA LNPs was determined using the qNano Gold tuneable resistive pulse sensing device. (C) dmLNP-siRNAnanoparticle characteristics including size, polydispersity (PDI), surface charge (zeta potential), and siRNA encapsulation efficiency. (D) dmLNP-siRNA biodistribution was determined in C57/BL6 mice that received DiD-labeled dmLNP-siRNA nanoparticles at 1 mg/kg siRNA dose or PBS vehicle control via retro-orbital (RO) route. 24 h after injection, mice were euthanized and the lung, liver, and spleen were removed. Organs were imaged for DiD fluorescence using a LagoX small animal imaging machine at excitation and emission wavelength of 640 and 690 nm, respectively. (E) Quantitative analysis of DiD fluorescence in each organ with n = 2 mice per treatment group.