Table 3.

A. Abbott Alinity m SARS-CoV-2 Assay Precision.

Concentration	Day	CN	Mean CN	SD	%CV	Instrument
1000 virus copies/mL	1	33.48	33.0	0.5	1.50%	Alinity m #1
	2	33.10
	3	32.48
200 virus copies/mL	1	35.18	35.4	0.2	0.60%
	2	35.48
	3	35.55
Negative	1	NDET	N/a	N/a	N/a
	2	NDET
	3	NDET
1000 virus copies/mL	1	33.41	33.1	0.3	0.80%	Alinity m #2
	2	33.07
	3	32.92
200 virus copies/mL	1	36.22	35.5	0.7	1.90%
	2	34.99
	3	35.16
Negative	1	NDET	N/a	N/a	N/a
	2	NDET
	3	NDET
Table 3B. UW LDT SARS-CoV-2 Assay Precision Comparison
Concentration	Day	N1 CN	N2 CN	N1 Mean FCN	N2 Mean FCN	N1 SD	N2 SD	N1%CV	N2%CV	Extraction Instrument
1000 virus copies/mL	1	34.19	35.28	34.65	35.16	0.40	0.17	1.16	0.48	MagNA Pure 96 #1
	2	34.95	34.97
	3	34.80	35.23
200 virus copies/mL	1	36.81	38.02	36.36	37.34	1.82	0.96	5.00	2.57
	2	34.36	36.66
	3	37.91	NDET
Negative	1	NDET	NDET	N/a	N/a	N/a	N/a	N/a	N/a
	2	NDET	NDET
	3	NDET	NDET
1000 virus copies/mL	1	33.76	35.79	33.89	35.44	0.32	0.51	0.95	1.44	MagNA Pure 96 #2
	2	33.66	35.67
	3	34.26	34.86
200 virus copies/mL	1	36.42	36.70	37.07	37.41	0.63	0.97	1.70	2.59
	2	37.11	37.02
	3	37.68	38.51
Negative	1	NDET	NDET	N/a	N/a	N/a	N/a	N/a	N/a
	2	NDET	NDET
	3	NDET	NDET

Abbreviations: CN, cycle number; SD, standard deviation,%CV, percent coefficient of variance, NDET, not detected; N/a, not applicable.

Different sample concentrations and a negative control (He-La cells) were run in triplicate over 72 h to evaluate SARS-CoV-2 assay precision between two separate Alinity m instruments.

Abbreviations: UW, University of Washington; LDT, laboratory developed test; CN, cycle number; SD, standard deviation,%CV, percent coefficient of variance, NDET, not detected; N/a, not applicable.

Different sample concentrations and a negative control (He-La cells) were run in triplicate over 72 h to evaluate the Alinity m SARS-CoV-2 assay precision in comparison to UW's LDT.