Table 1.

Primers, probe and qPCR conditions.

Name	Sequence (5′−3′)	Positions*
Primers:
Pri_IHU_N501Y_F1	ATCAGGCCGGTAGCACAC	22,980–22,997
Pri_IHU_N501Y_R1	AAACAGTTGCTGGTGCATGT	23,135–23,116
Probe (6FAM-labelled):
Pro_IHU_C_GB_1_MBP	CCACTTATGGTGTTGGTTACCAA	23,058–23,080

The qPCR was performed by adding 5 μL of extracted viral RNA to 15 μL of reaction mixture containing 5 μL of 4X TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher Scientific, Grand Island, NY, USA), 0.5 μL of forward primer (10 pmol/µL), 0.5 μL of reverse primer (10 pmol/µL), 0.4 μL of probe (10 pmol/µL), and 8.6 μL of water. PCR conditions are as follows: reverse transcription at 50 °C for 10 min, then a hold at 95 °C for 20 s followed by 40 cycles comprising a step at 95 °C for 15 s and a step at 60 °C for 60 s. This qPCR was run on a LC480 thermocycler (Roche Diagnostics, Mannheim, Germany).

*in reference to SARS-CoV-2 genome GenBank Accession no. NC_045512.2 (Wuhan-Hu-1 isolate). The nucleotide carrying the mutation is covered by the probe and underlined.