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. 2021 Apr 8;6(7):e140750. doi: 10.1172/jci.insight.140750

Figure 4. The application of PEPT2 inhibition increased LPS-induced acute lung inflammation and blocked Ac-PGP transport in patients with ARDS.

Figure 4

C56BL/6J mice were intratracheally (i.t.) treated with cefadroxil (Cef) and 100 μg LPS (P. aeruginosa). BAL was collected after treatment for total cell counts (A), MPO (B), and MMP-9 (C) levels. The level of Ac-PGP peptides in the BAL was analyzed by ESI-LC-MS/MS (D). Statistical analysis was performed using 1-way ANOVA and Tukey’s multiple comparison post test, n = 3–9 mice/group. All values represent mean ± SD. The transepithelial transport of Ac-PGP peptides in BAL of patients with ARDS in the apical-to-basal directions across polarized H441 cell monolayers was measured with or without cefadroxil by ESI-LC-MS/MS (E). Data (mean ± SD) are expressed with 4–5 wells per group and were analyzed by Mann-Whitney test.