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. 2021 Apr 8;6(7):e143283. doi: 10.1172/jci.insight.143283

Figure 3. Testing optimized CD29 barcoding system.

Figure 3

(A) Using 112Cd-, 113In-, 114Cd-, 115In-, and 116Cd-conjugated anti-CD29 and anti-CD98 antibodies, 10 unique barcodes were established for both anti-CD29 and anti-CD98 utilizing a 5-choose-3 barcoding strategy. MC38 and KPC cancer cell lines were stained using the aforementioned antibodies at a concentration of 0.25 μg/100 μL. (B) Ten-plexed MC38 and 10-plexed KPC samples were debarcoded using their respective gating hierarchies back to their original individual samples. Representative gating schema for the MC38 batch stained with anti-CD29 barcodes are shown. (C and D) Distribution of individual anti-CD29 barcodes (C) and anti-CD98 barcodes (D) yielded from the debarcoding process of MC38 and KPC samples. Both CD29 and CD98 barcoding systems resulted in a fairly even distribution of all 10 barcodes in MC38 and KPC samples.