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. 2021 Apr 8;6(7):e136911. doi: 10.1172/jci.insight.136911

Figure 4. Effect of ORMDL3 on ASM TPM expression and ASM proliferation.

Figure 4

(A). Levels of TPM1, TPM2, TPM3, and TPM4 mRNA were quantitated by RT-qPCR in ASM derived from WT and hORMDL3Myh11eGFP-cre mice (n = 3–4/group). (B) Levels of Myh10 and (C) vimentin mRNA were quantitated by RT-qPCR in ASM derived from WT and hORMDL3Myh11eGFP-cre mice (n = 3–4/group). (D) BrdU proliferation assay of ASM from hORMDL3Myh11eGFP-cre (n = 3/group) or (E) ASM from WT mice (n = 3/group) knocked down with TPM1 siRNA, TPM4 siRNA, or control scrambled siRNA. *P < 0.05 compared with control scrambled siRNA. P values are results of unpaired 2-tailed t tests (A–C) or 1-way ANOVA with Tukey’s post hoc test (D and E).