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The human SPL (hSPL) cDNA and the hSPL-tRFP cDNA were cloned into pAAV-MCS, packaged in AAV8, and used to transduce SPLIS skin fibroblasts. (A) Immunoblot of hSPL in whole cell extracts of SPLIS fibroblasts treated with vehicle (Ctrl), AAV-SPL, an equal volume of AAV-SPLtRFP, or a 5-fold higher volume of AAV-SPL-tRFP. (B) SPL activity in extracts corresponding to the samples described in A.
