Figure 12. Durability of AAV-SPL.

(A) Abundance of liver mSPL and hSPL are shown by immunoblot of livers from WT, KO, and long-lived AAV-SPL–treated KO mice (AAV). Blots were probed using anti-mSPL, which detects mSPL and crossreacts with hSPL, and using anti-hSPL, which is specific for hSPL. WT liver expresses mSPL but not hSPL. Untreated KO does not express either protein. Liver of the AAV-SPL–treated KO mouse euthanized at 11.5 months of age shows abundant hSPL, detected by both antibodies. GAPDH is a loading control. (B) SPL activity measured in livers of WT, KO, and long-lived AAV-SPL–treated KO (AAV) mice (n = 3/group) euthanized at 11 months of age. Using unpaired t test with Bonferroni’s correction, there were NSDs between the groups.