Fig. 1. Multiple alignment of partial amino acid sequences including three key residues of Emi2 and other reported hexokinases from S. cerevisiae .

　Multiple sequence alignment of Emi2 and the other characterized hexokinases, Hxk1, Hxk2, and Glk1, from S. cerevisiae . The sequences were aligned using Clustal W. ²⁰⁾ Strictly conserved residues are highlighted in black. The similar residues are shown in gray. Three conserved key residues, Asp-211, Ser-165, and Lys-110 of Emi2, predicted to be important for catalysis and for binding to glucose and ATP, respectively, are indicated by black, gray and white triangles below the sequences. The figure was created using BoxShade software.