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. 2020 Nov 20;67(4):103–109. doi: 10.5458/jag.jag.JAG-2020_0007

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2020 by The Japanese Society of Applied Glycoscience

This is an open-access paper distributed under the terms of the Creative Commons Attribution Non-Commercial (by-nc) License (CC-BY-NC4.0: https://creativecommons.org/licenses/by-nc/4.0/).

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Fig. 2. — M, molecular mass markers; Cell free, the cell free extract of BL21(DE3) expressing rEmi2; Unbound, the unbound fraction eluted from the column; Eluate 1 and 2 , the fraction eluted from the column by imidazole at 5 and 10 mM, respectively; Purified rEmi2 , the eluate from the column by imidazole at 250 mM; Cell free (empty), the cell free extract of BL21(DE3) carrying the empty pCold II vector.