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. 2020 Sep 12;28(5):442–454. doi: 10.1038/s41417-020-00226-z

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© The Author(s), under exclusive licence to Springer Nature America, Inc. 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Adenovirus Ad5/3-Luc1 (a, c) and TILT-123 (b, d) were incubated with human lymphocytes at 10 VP/cell (3 × 10e7 VP/ml with 3 × 10e6 cells/ml) (a, b) and with erythrocytes at 0.036 VP/cell (5 × 10e9 VP/ml and 1.8 × 10e8 cells/ml) (c, d) at 37°C. After 30 min incubation, cellular fraction and supernatant were isolated through centrifugation at 2000 × g for 10 min. Cellular fraction was washed five times and after each wash a sample from the supernatant and pellet was collected and analyzed through qPCR. Viral copy number was normalized against amount of genomic DNA in the sample, determined by the expression level of human β-actin. Data are presented as mean + SEM. Adenovirus particles associated with cell fraction (black bar) and supernatant (Sup, gray bars). No wash; first centrifugation after incubation, 1x wash; washed with PBS once, 2x wash; washed twice, 3x wash; 4x wash; 5x wash; washed three, four and five times, respectively.