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. 2020 Sep 12;28(5):442–454. doi: 10.1038/s41417-020-00226-z

Fig. 2. Adenovirus transduction and tumor cell killing potential after interaction with lymphocytes and erythrocytes.

Fig. 2

Ad5/3-Luc1 (a, b) and TILT-123 (c, d) were incubated at 37°C for 30 min with human lymphocytes (a, c) at 10 VP/cell or with human erythrocytes (b, d) at 0.036 VP/cell. After incubation, samples were centrifuged, resuspended and then 1:1.7, 1:2.7, and 1.6.7 dilutions of cell–adenovirus mixture was added to A549 monolayer. For one group, cell–adenovirus mixture was washed three times with PBS at 2000 × g for 10 min and then 1:2.7 dilution of cell suspension was added to A549 monolayer. Luciferase expression was measured after 48 h (a, b). Tumor-killing ability of TILT-123 as such (0.1–100 VP/cell) or when delivered with lymphocytes (c) or erythrocytes (d) was analyzed on day 3 with cytotoxicity (MTS) assay on A549 cells. Data are presented as mean + SEM. No wash; first centrifugation after incubation, 3x wash; washed with PBS three times.