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. Author manuscript; available in PMC: 2022 Jun 1.
Published in final edited form as: Pain. 2021 Jun 1;162(6):1705–1721. doi: 10.1097/j.pain.0000000000002179

FIGURE 1.

FIGURE 1.

CFA injection induces mechanical allodynia, thermal hyperalgesia, and paw swelling for most mice for at least six weeks.

A: Paw withdrawal thresholds in the von Frey assay for mechanical allodynia of mice injected with saline or CFA and tested weekly for six weeks (n=9,9).

B: Paw withdrawal latencies in the Hargreaves assay for thermal hyperalgesia of mice injected with saline or CFA and tested at 24 hours, 72 hours, and weekly for six weeks (n=9,9).

C: Paw thickness and swelling as measured by caliper of mice injected with saline or CFA and tested at 24 hours, 72 hours, and weekly for six weeks (n=9,9).

D: Paw withdrawal latencies in the Hargreaves assay for thermal hyperalgesia of mice injected saline or CFA, and tested four or six weeks later in behavioral tests (n=80 SAL, n=46 CFA 4-weeks, n=37 CFA 6-weeks). Gray shading indicates overlap between SAL and CFA values.

E: Paw withdrawal latencies in the Hargreaves assay for thermal hyperalgesia of mice injected saline or CFA, and tested four or six weeks later in behavioral tests, with REC mice represented distinct from CFA (n=80 SAL, n=8 REC 4-weeks, n=38 CFA 4-weeks, n=15 REC 6-weeks, n=22 CFA 6-weeks).