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. Author manuscript; available in PMC: 2022 Jun 1.
Published in final edited form as: Pain. 2021 Jun 1;162(6):1705–1721. doi: 10.1097/j.pain.0000000000002179

FIGURE 4.

FIGURE 4.

Inflammatory pain persisting for four weeks may alter exploratory behavior and passive coping strategy, when testing order is reversed. White-filled data points in the REC group indicate where REC n<4 and was not compared to SAL and CFA in ordinary one-way ANOVAs or Kruskall-Wallis tests. White-filled data points in the SAL group indicate mouse with a putative paw lesion. Lines compare SAL vs. CFA, and SAL vs. REC, line ending in a bracket compares SAL vs. CFA+REC together.

A: Schematic of experimental design.

B: Paw withdrawal thresholds in the Hargreaves assay for thermal hyperalgesia by mice injected with CFA and sustaining thermal hyperalgesia for four weeks (n=17), compared with mice injected with CFA which recovered (n=7), and with mice injected with saline (n=23).

C: Entries into the center of the OFT by mice injected with CFA and sustaining thermal hyperalgesia for four weeks (n=17), compared with mice injected with CFA which recovered (n=7), and with mice injected with saline (n=23).

D: Time spent in the center of the OFT by mice injected with CFA and sustaining thermal hyperalgesia for four weeks (n=17), compared with mice injected with CFA which recovered (n=7), and with mice injected with saline (n=23).

E: Distance traveled in the open field by mice injected with CFA and sustaining thermal hyperalgesia for four weeks (n=17), compared with mice injected with CFA which recovered (n=7), and with mice injected with saline (n=23).

F: Time spent immobile in the FST by mice injected with CFA and sustaining thermal hyperalgesia for four weeks (n=11), compared with mice injected with CFA which recovered (n=1), and with mice injected with saline (n=12).

G: Latency to immobility in the FST by mice injected with CFA and sustaining thermal hyperalgesia for four weeks (n=11), compared with mice injected with CFA which recovered (n=1), and with mice injected with saline (n=12).

H: Entries into the open arms of the EZM by mice injected with CFA and sustaining thermal hyperalgesia for four weeks (n=6), compared with mice injected with CFA which recovered (n=6), and with mice injected with saline (n=10).

I: Time spent in the open arms of the EZM by mice injected with CFA and sustaining thermal hyperalgesia for four weeks (n=6), compared with mice injected with CFA which recovered (n=6), and with mice injected with saline (n=10).