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. Author manuscript; available in PMC: 2022 Jun 1.
Published in final edited form as: Pain. 2021 Jun 1;162(6):1705–1721. doi: 10.1097/j.pain.0000000000002179

FIGURE 5.

FIGURE 5.

Measures of exploratory behavior do not consistently or reliably correlate between two tests of behavior in a novel, anxiogenic environment.

A: Equation used to calculate z-score for individual measurements of entries into open arms of EZM, time spent in open arms of EZM, entries into center of OFT, and time in center of OFT.

B: Key for correlations. Each box contains the p-value of the strength of correlation between the measurements aligned with its location. Gray boxes contain significant p-values for saline groups. Black boxes contain insignificant p-values. Red boxes contain significant p-values for CFA groups.

C: In saline-injected mice tested four weeks later first in the EZM and later in the OFT, time spent in the open arms of the EZM correlates only to entries into the open arms of the EZM and entries into the center of the OFT. Time spent in the center of the OFT does not correlate to any other measure (n=10).

D: In CFA-injected mice tested four weeks later first in the EZM and later in the OFT, time in the center of the OFT correlates to entries into the center of the OFT and entries into the open arms of the EZM. Time in the open arms of the EZM does not correlate to time in the center of the OFT or entries into the open arms of the EZM (n=10).

E: In saline-injected mice tested four weeks later first in the OFT and later in the EZM, entries into the center of the OFT correlates to entries into the open arms of the EZM and time spent in the open arms of the EZM. Time in the center of the OFT does not correlate to any other measure (n=10).

F: In CFA-injected mice tested four weeks later first in the OFT and later in the EZM, no behavioral measures correlate (n=6).

G: In saline-injected mice tested six weeks later first in the EZM and later in the OFT, no behavioral measures are associated except when CFA+REC mice are grouped back together, in which case time and entries into the center of the OFT are significantly correlated (n=11).

H: In CFA-injected mice tested first six weeks later in the EZM and later in the OFT, entries into and time spent in the open arms are significantly correlated, and entries into and time spent in the center of the OFT are significantly correlated (n=6). These significant associations persist when CFA+REC mice are grouped back together (n=10).