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. Author manuscript; available in PMC: 2022 May 10.
Published in final edited form as: Curr Biol. 2021 Mar 10;31(9):1903–1917.e6. doi: 10.1016/j.cub.2021.02.015

Figure 1. A bias in epithelial orientation emerges along the long axis of 4-week-old inguinal mammary glands.

Figure 1.

A) Schematic of mouse mammary glands (inspired by57). B) Representative fluorescent image of a mammary gland labeled with Hoechst 33342 (left; scale bar represents 1 mm), skeleton of epithelium (middle), and visualization of branch orientation (right). C) Representative fluorescent images of no. 4 inguinal mammary glands at different stages of pubertal development labeled with Hoechst 33342 (scale bar represents 5 mm) and D) corresponding skeletons (scale bars represent 1 mm). E) Orientation and F) alignment fraction of epithelium at different stages of pubertal development. The dashed red line represents the alignment fraction corresponding to an epithelium without an orientation bias. G) Number of epithelial branches, H) distribution of branch length, and I) average branch length at different stages of pubertal development. Data are represented as mean ± SD. *p ≤ 0.05 and ***p ≤ 0.001. See also Figure S1.