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. Author manuscript; available in PMC: 2022 May 10.
Published in final edited form as: Curr Biol. 2021 Mar 10;31(9):1903–1917.e6. doi: 10.1016/j.cub.2021.02.015

Figure 6. Collagen-rich ECM is deposited along the flanks and at the cleft site of bifurcating TEBs.

Figure 6.

A) Representative fluorescent images of 150-μm-thick sections of TEBs during extension, cleft formation, and bifurcation and B) corresponding schematic of the pattern of collagen accumulation. C) Representative fluorescent images of 150-μm-thick sections of bifurcating TEBs with different angles of bifurcation. D) Area of collagen accumulation along the flanks and at the cleft site as a function of the angle of bifurcation; n=6 bifurcations. E) Schematic of hypothesized model in which the local extent of collagen accumulation regulates the angle of TEB bifurcation. Tissues are stained with Hoechst 33342 to label nuclei (magenta) and an antibody against type I collagen (green). Scale bars represent 100 μm or 50 μm for insets.