| Antibodies |
| Mouse monoclonal anti-collagen 1 |
Thermo Fisher Scientific |
Cat#MA1–26771 RRID:AB_2081889 |
| Alexa Fluor 488 goat anti-mouse secondary antibody |
Thermo Fisher Scientific |
Cat#A-11001 |
| Bacterial and Virus Strains |
| BL21(DE3) |
Addgene |
N/A |
| Biological Samples |
|
|
| Chemicals, Peptides, and Recombinant Proteins |
| Matrigel (growth factor reduced; protein concentration 8.4 mg/ml) |
Corning |
Cat#354230 |
| Bovine, Type I collagen with intact telopeptide |
Advanced BioMatrix |
Cat#5026 |
| Hepatocyte growth factor |
Sigma-Aldrich |
Cat#H9661 |
| Fetal bovine serum |
Gemini Bioproducts |
Cat#100–106 |
| Calf serum |
R&D Systems |
Cat#S11450
|
| Dexamethasone |
Sigma-Aldrich |
Cat#D2915 |
| 3- isobutyl-1-methylxanthine |
Sigma-Aldrich |
Cat#I7018 |
| Ni-NTA resin |
Qiagen |
Cat#30230 |
| Imidazole |
Sigma-Aldrich |
Cat#I2399 |
| Lysozyme |
Sigma-Aldrich |
Cat#L6876 |
| TRIS HCl |
Sigma-Aldrich |
Cat#T5941 |
| Triethanolamine |
Sigma-Aldrich |
Cat#90278 |
| Quadrol® |
Sigma-Aldrich |
Cat#122262 |
| Tissue-Tek® O.C.T. compound |
Sakura |
Cat#4583 |
| Permount™ mounting medium |
Fisher Scientific |
Cat#SP15–100 |
| (3,3,3-Trifluoropropyl)trichlorosilane, 97% |
Alfa Aesar |
Cat#L16670
|
| Critical Commercial Assays |
| Deposited Data |
| Experimental Models: Cell Lines |
| Normal EpH4 mouse mammary epithelial cells |
ATCC |
|
| 3T3-L1 preadipocytes |
ATCC |
CL-173 RRID:CVCL_0123 |
| Experimental Models: Organisms/Strains |
| Mouse: CD-1 |
Charles River Laboratories |
Strain code: 022 |
| Mouse: C57BL/6J |
The Jackson Laboratory |
Jax: 000664 |
| Oligonucleotides |
| Recombinant DNA |
| pET28a-mCherry-CNA35 |
64 |
Addgene Plasmid #61607 RRID:Addgene_61607 |
| Software and Algorithms |
| Imaris v9.5.1 |
Bitplane |
https://imaris.oxinst.com/ |
| GraphPad Prism v8.0 and 9.0 |
GraphPad |
https://www.graphpad.com/ |
| MATLAB vR2015b and vR2019a |
MathWorks |
https://www.mathworks.com/products/matlab.html |
| Mechanical model of epithelial branching |
28 |
N/A |
| Branching and annihilating random walk model |
22 |
N/A |
| ETE3 Python toolkit v 3.1.1 |
67 |
http://etetoolkit.org/ |
| gnuplot v5.2 |
N/A |
http://www.gnuplot.info/ |
| Gmsh v4.5.6 |
70 |
https://gmsh.info/ |
| ParaView v5.7.0 |
76 |
https://www.paraview.org/ |
| FEniCS v2019.1.0 |
73 |
https://fenicsproject.org/book/ |
| Other |
