KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| anti-MRTFB antibody | Bethyl Laboratories | Cat#A302-768A |
| anti-MRTFA antibody | Santa Cruz Biotechnology | Cat#sc-390324 |
| Anti-GAPDH antibody | Cell Signaling | Cat#2118L |
| IRDye 680RD Goat anti-Rabbit IgG Secondary Antibody | Licor Biosciences | Cat#926-68071 |
| IRDye 800CW Goat anti-Mouse IgG Secondary Antibody | Licor Biosciences | Cat#926-32210 |
| APC conjugated anti-CD8a antibody | Tonbo Biosciences | Cat#20-0081 |
| eFluor660 conjugated anti-Lamp1 antibody | eBiosciences | Clone: 1D4B; Cat#50-1071-82 |
| PE conjugated anti-TNF | BioLegend | Cat#506306 |
| PE/Cy7 conjugated anti-IFNγ | BioLegend | Cat#505826 |
| PerCP-Cy5.5 conjugated anti-NK1.1 antibody | eBioscience | Cat#45-5941-82 |
| Brilliant Violet 786-labeled anti-Lamp1 mAb | BD Horizon | Cat#563869; Clone: SJ25C1 |
| ECD-labeled anti-CD56 mAb | Beckman Coulter | Cat#A82943 |
| BV650-labeled anti-CD3 mAb | BD Horizon | Cat#563852; clone:UCHT1 |
| PE-labeled anti-NKG2D | BioLegend | Cat#320806; clone:1D11 |
| Anti-ATP1A1 antibody | Cell Signaling Technology | Cat#3010S |
| Anti-Histone H3 antibody | Cell Signaling Technology | Cat#9715 |
| Anti-H2Kb antibody | Abcam | Cat#ab93364 |
| Anti-GM130 antibody | BD Biosciences | Cat#610822 |
| Phospho Paxillin (Tyr118) Antibody | Cell Signaling Technology | Cat#2541S |
| APC-labeled anti-Fas | Biolegend | Cat#152603 |
| PE anti-mouse H-2Kb Antibody | Biolegend | Cat#116508; Clone: AF6-88.5 |
| PE Anti-Mouse MHC Class I (H-2Db) | eBiosciece | Cat#12-5999-81 |
| mouse NKG2D-Fc | Joseph Sun (Madera et al., 2016) | N/A |
| PE anti-human IgG | Jackson | Cat#109-116-098 |
| PE-labeled anti-MICA/B | BD Pharmingen | Cat#558352; clone: 6D4 |
| PE-Cy5-labeled anti-HLA-ABC | BD Pharmingen | Cat#555554; clone: G46-2.6 |
| InVivoMab anti-mouse CD8α | Bio X Cell | Cat#BE0004-1; Clone: 53-6.7 |
| Anti-Asialo GM1 | Wako Chemicals | Cat#986-10001 |
| InVivoPlus rat IgG2a isotype control | Bio X Cell | Cat#BP0089; Clone: 2A3 |
| InVivoPlus anti-mouse PD-1 (CD279) | Bio X Cell | Cat#BP0146: Clone: RMP1-14 |
|
InVivoPlus anti-mouse CTLA-4 (CD152) Anti-CTLA-4 Mouse Monoclonal Antibody |
Bio X Cell | Cat#BP0164; Clone: BP0164 |
| InVivoPlus mouse IgG2b isotype control | Bio X Cell | Cat#BP0086; Clone: MPC-11 |
| Anti-CD8 rabbit monoclonal | Cell Signaling Technology | Cat#98941 |
| Anti-NKp46 goat polyclonal | R&D Systems | Cat#AF2225 |
| Biological Samples | ||
| Human breast and lung cancer samples | MSK Department of Pathology | N/A |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Recombinant Human sFas Ligand (FasL) | PeproTech | Cat#310-03H |
| Live/Dead Fixable Near IR Dead Cell Stain Kit | Molecular Probes | Cat#L34976 |
| GolgiSTOPTM | BD | Cat#554724 |
| CellTrace Violet | Thermo Fisher | Cat#C34557 |
| Staurosporine | Cell Signaling Technology | Cat#9953 |
| Recombinant Murine TNF-α | PeproTech | Cat#315-01A |
| Recombinant Mouse Granzyme B | Biolegend | Cat#554804 |
| R&D Systems™ Mouse Active Cathepsin C/DPPI Recombinant Protein | R&D Systems™ | Cat#2336CY010 |
| Alexa Fluor™ 594 Phalloidin | Thermo Fisher Scientific, Invitrogen | Cat#A12381 |
| DAPI | Thermo Fisher Scientific | Cat#D1306 |
| CellMask™ Orange Plasma membrane Stain | Invitrogen | Cat#C10045 |
| mouse interferon gamma | BioLegend | Cat#575304 |
| Live/Dead Fixable Aqua Dead Cell Stain Kit | ThermoFisher | Cat#L34957 |
| BD Cytofix/Cytoperm Plus Kit (with BD GolgiPlug) | BD | Cat#555028 |
| RNeasy Mini Kit | Qiagen | Cat#74106 |
| Human interleukin 2 | NCI Biological Resources Branch Repository | N/A |
| NK Cell Isolation Kit, mouse | Miltenyi Biotec MACS | Cat#130-115-818 |
| D-Luciferin, potassium salt | Goldbio | Cat#LUCK-1G |
| Fibronectin, Bovine Plasma | Millipore Sigma | Cat#341631-5MG |
| Fura-2, AM, cell permeant | Thermo Fisher Scientific | Cat#F1221 |
| Doxycycline hyclate | Sigma-Aldrich | Cat#PHR1145-1G |
| Ki67 | Cell Signaling Technologies | Cat#9129 |
| RosetteSepTM Human NK Cell Enrichment Cocktail | Stem Cell Technologies | Cat#15025 |
| IL-2 | Proleukin, Prometheus | Cat#65483011607 |
| Critical Commercial Assays | ||
| CellTiter-Glo® Luminescent Cell Viability Assay Kit | Promega | Cat#G7570 |
| LDH Cytotoxicity Detection Kit | Takara | MK401 |
| Caspase-Glo® 3/7 Assay System | Promega | Cat#G8090 |
| Deposited Data | ||
| GEO | NCBI | GSE164860 |
| cBioportal | Cerami et al., 2012; Gao et al., 2013 | |
| TCGA PanCancer Atlas database | Ellrott et al., 2018; Gao et al., 2018; Hoadley et al., 2018; Liu 821 et al., 2018; Sanchez-Vega et al., 2018; Taylor et al., 2018 | |
| Genomics Data Commons Data Portal | https://portal.gdc.cancer.gov/ | |
| Tumor Immune Dysfunction and Exclusion | http://tide.dfci.harvard.edu/ | |
| Experimental Models: Cell Lines | ||
| E0771 | CH3 Biosystems | 94A001 |
| B16F10 | Wolchok Lab | N/A |
| MCF-7 | ATCC | HTB-22 |
| MDA-MB-231 | Massagué Lab | N/A |
| Experimental Models: Organisms/Strains | ||
| Mouse:C57BL/6J | The Jackson Laboratory | Cat#000664 |
| Mouse:OT1:B6.129S6-Rag2tm1Fwa Tg(TcraTcrb)1100Mjb | Taconic Farms | Cat#2334 |
| Recombinant DNA | ||
| p3XFLAG-MKL2 | Ron Prywes (Selvaraj et al., 2003) | Addgene#27175 |
| pRetroX-Tight-Hygro vector | Takara Bio | Cat#631034 |
| pLVX-TetON-Advanced | Takara Bio | Cat#632162 |
| pTetON-DHFRdd-SpvB; CMV-Cherry | Brad Zuchero (Harterink et al., 2017) | Addgene#89463 |
| pLKO.1-MKL1/2 shRNA | Ron Prywes (Lee et al., 2010) | Addgene#27161 |
| MSCV-LifeAct-GFP | Le Floc’h et al., 2013 | N/A |
| MSCV-GFP | Le Floc’h et al., 2013 | N/A |
| TGL | Ponomarev et al., 2004 | N/A |
| pRevTRE MKL1 | Ron Prywes (Muehlich et al., 2008) | Addgene#19846 |
| Software and Algorithms | ||
| Living Image software v.2.50 | Perkin Elmer | N/A |
| FlowJo software v10.7.1 | BD | N/A |
| Imaris software v8 | Bitplane | N/A |
| CaseViewer | 3D Histech | N/A |
| Aperio Imagescope | Leica Biosystems | N/A |
| Igor Pro | Wavemetrics | N/A |
| Prism v8 | GraphPad | N/A |
| rnaStar aligner | Dobin et al., 2013 | N/A |
| HTSeq | EMBL | www-huber.embl.de/users/anders/HTSeq |
| DESeq | EMBL | www-huber.embl.de/users/anders/DESeq |
| DAVID 6.8 Functional Annotation Tool | Huang da et al., 2009 | N/A |
| Halo image analysis software | Indica Labs | N/A |