Female EC-MR intact (EC-MR-WT) and EC-MR-KO mice were fed normal (lean) or high fat diet (obese) for 20 weeks and endothelial-dependent vasodilation was measured ex vivo using acetylcholine (ACh). (A) In lean females, there is no difference in vasodilation comparing EC-MR-WT (closed circles) versus EC-MR-KO (open circles). (B) In obese females, vasodilation is improved in EC-MR-KO versus EC-MR-intact. *p<0.05, ***p<0.001, 2-way repeated measures ANOVA, Bonferroni post hoc.