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. Author manuscript; available in PMC: 2022 Jun 1.
Published in final edited form as: Hypertension. 2021 May 3;77(6):2117–2126. doi: 10.1161/HYPERTENSIONAHA.120.16911

Figure 3. Aldosterone Inhibits Estrogen-Induced eNOS Phosphorylation via MR in Endothelial Cells.

Figure 3.

(A) EAhY endothelial cells stably expressing ERα were treated with scrambled control siRNA or MR siRNA for 48 hours and MR knock down was demonstrated by immunoblotting. (B-C) Cells were treated for 20 minutes with vehicle, 10 nM aldosterone (Aldo), 10 nM estradiol (E2) or E2+Aldo. Ratio of phospho-eNOS to total eNOS was quantified by immunoblotting: (B) in control cells with MR or (C) with MR knock down. *p<0.05, **p<0.01, ****p<0.0001, N=3, one-way ANOVA with Bonferroni post hoc.